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Return to Case it Home Page. This manual includes case descriptions and instructions for case studies included with the Case It! Adult wants real sex Bergland the tutorials for more detailed instructions on use of the software simulation.

For the Instructor's version, including additional background information and keys to cases, contact Mark Bergland at mark. Contact mark.

This project was supported, in part, by the National Science Foundation. Opinions expressed are those of the authors and not necessarily those of the Foundation.

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CopyrightUniversity of Wisconsin-River Falls. Case It! The overall goal is to develop a framework for collaborative case-based learning in molecular biology.

The Case It software is an open-ended simulation which reads nucleotide or amino acid sequence files, and includes methods for analyzing Bervland restriction digestion and mapping, polymerase chain reaction PCRDNA electrophoresis, Southern blotting and dot blotting, microarray analysis and proteins protein electrophoresis, Western blotting, ELISA.

Detailed tutorials that describe the various features and the steps for using them can be accessed from the Case It home page http: Case It software can be downloaded from the Adult wants real sex Bergland It web site at no cost to educators or students. Most of the cases described here were developed for Adult wants real sex Bergland in introductory undergraduate biology classes to help students address concepts and issues in molecular biology, but they can be dex to a variety of educational settings.

These cases guide students through the analysis steps and then provide focused questions to prompt interpretation and application of the results. Each case description includes the case scenario and instructions for analyzing the case, as well as background information and discussion questions.

The cases can be presented to students using this format, having them read the background information and perhaps do some Bdrgland research, then carry out the analysis, interpret the results and discuss the significance and the issues raised.

Alternatively, instructors can edit the cases to People looking to fuck in shreveport or Adult wants real sex Bergland information as appropriate for the backgrounds of students and the course objectives.

Students may be required to: In addition to using these cases and sequences, instructors may develop their own cases using DNA sequences obtained from GenBank or elsewhere see "Building your own case study" below.

Sequences, restriction Adult wants real sex Bergland rral, probes, primers and antibodies all are editable text files. Case development also can be assigned to students in more advanced biology courses. The student-designed cases then can be subjected to peer review via poster presentations, etc. The DNA and protein sequences for the cases described here are located in the Cases folder that is downloaded with the Case It! Software simulation.

The necessary Adlt, probes, antibodies, or proteins for a particular case will be located in the same folder as the DNA sequences. The keys to Chesapeake japanese massage cases are available online to instructors — contact Mark Bergland mark. Genetic diseases are caused by mutations, or changes in the DNA, which result in loss of function or altered Adult wants real sex Bergland of a protein.

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Adult wants real sex Bergland These changes in the DNA can be detected, wwants in the absence of disease symptoms, by isolating DNA from the patient and using various molecular biology lab techniques to detect the mutation.

The following cases illustrate different types of DNA mutations associated with human genetic diseases, as well as different lab techniques, including restriction enzyme digestion and restriction fragment length polymorphism RFLPSouthern blot, polymerase chain reaction PCRand dot reql.

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Each case scenario indicates which technique to use, and the necessary sequences DNA samples, primers, probes are found in the case folder. Note that the techniques used here do not necessarily represent what would be done in a clinical laboratory - PCR and DNA Adult wants real sex Bergland are increasingly being used for diagnosis, but these cases are intended to illustrate the variety of Bbw seeks sexdate used to detect DNA differences.

Wanta value judgment regarding individuals who have Adult wants real sex Bergland the disease-associated mutations is intended or implied. Sickle cell anemia Background: Sickle cell anemia is a disease of red blood cells. It is caused by a mutation in the Brgland gene.

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A single base change results in a single amino acid substitution. This mutation causes the hemoglobin to change its conformation to a more elongated form under certain conditions, distorting the red blood cells and impairing their ability to carry oxygen.

BibMe Free Bibliography & Citation Maker - MLA, APA, Chicago, Harvard. This manual includes case descriptions and instructions for case studies included with the Case It! v7 download. See the tutorials for more detailed instructions on use of the software simulation. Click here for a PDF version of this Resource Manual. For the Instructor's version, including additional background information and keys to cases, contact Mark Bergland at [email protected] The stress hormone, cortisol, is public health enemy number one. Scientists have known for years that elevated cortisol levels: interfere with learning and memory, lower immune function and bone.

Sickle cell anemia is considered a recessive trait, since both chromosomes have to carry the Adlut Adult wants real sex Bergland order for the full blown disease symptoms to appear. The sickle cell mutation also eliminates a restriction enzyme site - the recognition site for the enzyme MstII.

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The presence qants absence of the sickle cell mutation can be determined based on the size of the fragment identified by the probe. Case A: Steve and Martha are expecting their second child.

They know that sickle cell anemia runs in both of their families. They want to know whether this child could be affected.

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Neither they nor their year-old daughter, Sarah, Adult wants real sex Bergland shown any symptoms of the disease. They decide to have DNA tests to determine the status of the fetus, as well as to find out whether they in fact are carriers of the disease gene. Then run Adult wants real sex Bergland Southern blot, using the probe corresponding to the region of the hemoglobin gene mutated in sickle cell anemia, to determine the genotype of each individual.

Case B: Mattie has just returned form the hospital Knutsford girls for sex visiting KC, her favorite nephew. She and her family are already grieving the loss they know is coming. She feels as helpless for them as she does for KC.

Josiah shook her up when he blurted out, during a period of overwhelming stress, that if they had known ahead of time, perhaps they would have chosen a different route, and that she should get tested to avoid the same suffering.

Adult wants real sex Bergland knew it was the stress talking, and that Josiah would not trade any of his moments with KC, but maybe he was right about her. Maybe she should go into parenting with her eyes open. Audlt she should find out Bergpand she could bear a child with sickle Adult wants real sex Bergland anemia.

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Case C: Claudine and Andre Kasonga live in a small community in sub-Sahara Africa, surrounded by family and friends whose children frequently Adult wants real sex Bergland from malaria or sickle cell anemia. They themselves have both had Adutl succumb to each of these diseases. While they both appear to be fine, they are expecting their first child and wish to know how to prepare themselves.

They decide to get tested.

What is the connection between the malaria-carrying parasite and sickle cell anemia? Under what fetal genetic conditions would it make sense to move out of the area where malaria is endemic?

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What conclusions can you draw from the results? What options are available to the family?

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What issues are raised by this type of testing? Top of page.

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The sants of onset for symptoms watns generally years. The genetic basis of the disease wangs an amplification in a gene with an as yet unknown function. A triplet CAG is repeated times in asymptomatic individuals; having more than 50 repeats is associated Adult wants real sex Bergland disease symptoms.

This amplification can be detected by restriction wantd digestion and Southern blot analysis, since the size of the fragment bound by the probe is increased as a Berland of the amplification of the triplet repeat. Alternatively, PCR can be used to isolate the region containing the triplet repeats; the relative size of the repeat region Adult wants real sex Bergland be determined by running the PCR products on a gel.

For Southern blot: For PCR: A paternal uncle, age 66, appears to be unaffected by the disease. Susan wants to know if she inherited the mutated Sex message Itasca from her father so that she can prepare for that future if necessary. Her year old brother, John, also decides to be tested after talking with Adult wants real sex Bergland.

Click on the large gel fragment from one of the samples that is positive for Huntington's disease. The sequence of that DNA should be visible in the lower window if not, check the Sequence box above the window.

The entire sequence, just a portion containing the repeat region, can be sent for BLAST analysis the latter will work better if using fragments from the I wanna lick sum sweet pussy blot.

How many triplet repeats does each individual have? Note that trees built using these sequences are not meaningful. Josiah and Eldrea were worried about their year-old father. He was starting to act sometimes like this older brother, their uncle Theo.

Theo was 15 years older than their father and he had been recently diagnosed with Huntington disease. After speaking with the family physician they learned a diagnostic DNA test was available. They wanted to their father to have the test, and they decided they should take it themselves so that they can better prepare for their Adult wants real sex Bergland.

This manual includes case descriptions and instructions for case studies included with the Case It! v7 download. See the tutorials for more detailed instructions on use of the software simulation. Click here for a PDF version of this Resource Manual. For the Instructor's version, including additional background information and keys to cases, contact Mark Bergland at [email protected] increase your libido and sex drive in female and male really matters in a relationship, i was facing difficulties in all my sex life i was humiliated because of my small penis and premature. The stress hormone, cortisol, is public health enemy number one. Scientists have known for years that elevated cortisol levels: interfere with learning and memory, lower immune function and bone.

Forty-four year old Jerry resl haunted by the specter of Huntington disease. It took his grandmother, a favorite uncle, and now he sees signs of motor impairment in his year-old mother, Adult wants real sex Bergland.

He worries that he might have inherited the disease and wonders, too, if he may have passed it to any of his 3 children. After several late night family discussions, a date is set for Berglannd to provide samples for DNA testing.

While he is certain he and his mother should be tested, he wonders if his children are making the right choice.

The symptoms of Duchenne's muscular dystrophy DMD include progressive and severe skeletal muscle weakness. A common mutation associated with DMD is a deletion of Adult wants real sex Bergland or more Adult wants real sex Bergland in the dystrophin gene. These deletions can be detected by restriction enzyme digestion and Southern blotting using a combination of probes that will bind to multiple dystrophin exons.

Jean and Bill have three sons, ages 12, 8, and 7, and a daughter, age 6. The oldest son and daughter are healthy, but the two younger sons are exhibiting symptoms of muscle weakness consistent with early muscular dystrophy. She seeks DNA testing to determine whether her younger sons may have inherited the form of Housewives wants sex TX Houston 77065 dystrophin gene associated with Duchenne's muscular dystrophy DMD.

Based on the number and sizes of the fragments bound by the probe, determine the status of each of the individuals tested.